Out-licensing

Expression of Human Glutaminyl Cyclase in Escherichia coli System

Glutaminyl cyclase is a catalyst responsible for protein N-terminal pyroglutamate formation. This enzyme is very useful in the commercially large-scale production of bioactive hormones and anticancer drugs which need the N-terminal pyroglutamate residue for their biological activities. In addition, human glutaminyl cyclase is a possible drug target for the treatment of several human disorders, such as Alzheimer's disease.

Conclusion

We have developed an expression system that has a relatively higher level of the recombinant human glutaminyl cyclase. We provide a convenient method for separation and purification of human glutaminyl cyclase from Escherichia coli cell lysates. In contrast to previous expression systems (yeast and insect cells), our system is in low cost, and our separation and purification procedures needs only a short period.

Relevance/Opportunity

Commercially, glutaminyl cyclase may facilitate the large-scale production of clinically useful hormones and anticancer drugs in cases of that the N-terminal pyroglutamate is required in the biological functions of these proteins. Please enquire quoting reference no. 11A-940825 if you are interested in licensing partnerships.

Preclinical

US pending, TW pending