Out-licensing

Evaluating the Effect and Efficiency of Biological Active Ingredients on Wound Healing

Introduction/Background

Wound healing is a highly controlled, complex physiological process that requires the sophisticated co-operation, production and re-organization of cells involved in haemostasis, inflammation, immune defence, vascularisation, fibroplasias and epithelialisation. Each of these steps could affect this interactive process and lead to an altered healing process. Recent advances in cellular and molecular biology have greatly expanded our understanding of the biological processes involved in wound repair and tissue regeneration. Wound healing can be evaluated in vitro or in vivo. For biotechnological applications and clinical relevance, selection of an animal model for wound healing studies depends on a number of factors including availability, cost, ease of handling, anatomical and the functional similarity of target tissues to humans. Anatomically and physiologically, the pig skin system has been shown to be very similar to human skin, with human and pig skins possibly healing through physiologically similar processes.

Aims/Hypothesis

We have employed the pig skin tissue wound-healing system for evaluating a series of novel wound healing drugs.

Research

We have effectively and conveniently evaluated a series of drugs, purified compounds, and the potential synergy among these compounds for wound healing or other therapeutic activities, using tissue arrays on pig skin of a single animal as an simple test system. Direct gene transfer of genetic material into an internal wounded target cell culture, or epidermal cell site in optional combination with a multiple wound treatment tissue array, are particularly effective as a means of monitoring of healing effect in vivo. The method of the invention serves as a platform for evaluation a wide variety of active ingredients for skin wound healing.

Conclusion

Our invention provide an in vivo, skin tissue array system and improved employment of transgenic reporter assays may help to create high through put experimental systems that can aid studies of skin tissue-wounding, DNA vaccination and physiology and development of determine tissues. We can evaluate potential bioactivities of a battery of herbal extracts or compounds on wound healing and regulation of transgenic expression.

Relevance/Opportunity

Please enquire quoting reference no. 14T-890523 regarding licensing or codevelopment partnerships.

Preclinical